*****----------------Description of the open data provided in the frame of manuscript  "Effect of high energy excimer treatment of Ti based alloys: On cyto-compatibility and antibacterial properties"-----------------------*****
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Authors: 	Petr Slepička a, Silvie Rimpelová b, Šárka Havlíčková a, Tomáš Kovářík c, Jiří Martan c, Michal Procházka c, Petr Sajdl d, Nikola Slepičková Kasálková a 

Afilitaion: a Department of Solid State Engineering, The University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic 
	    b Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic
	    c New Technologies - Research Centre, University of West Bohemia, Univerzitní 8, 301 00 Plzeň, Czech Republic 
	    d Department of Power Engineering, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic



Contact informations: Petr Slepička: petr.slepicka@vscht.cz

Data manager or custodian: Filip Průša (prusaf@vscht.cz)

Data were collected: 08/2025-02/2026

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File name structure:

The following repository contains .rar folders which are distinguished accordingly to the chosen type of analysis.

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-   Analysis 1	(SEM)
		- Data are in .tiff named as Fig.1_pristine_10micron; Fig.1_pristine_30micron; Fig.1_pristine_100micron; Fig.1_laserTr_10micron; Fig.1_laserTr_30micron; Fig.1_laserTr_100micron
                                             Fig.2_pristine_10micron; Fig.2_pristine_30micron; Fig.2_pristine_100micron; Fig.2_laserTr_10micron; Fig.2_laserTr_30micron; Fig.2_laserTr_100micron
	                                     Fig.3_pristine_10micron; Fig.3_pristine_30micron; Fig.3_pristine_100micron; Fig.3_laserTr_10micron; Fig.3_laserTr_30micron; Fig.3_laserTr_100micron 
		
	- Data description of:  Methods used for acquiring/creation of data: scanning electron microscope (FIB-SEM LYRA3 GMU, TESCAN) 
				Summary of variables: WD = Working distance; DE = Detector; HV = High Voltage; MAG = Magnification
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-   Analysis 2	(EDS)
		- Data are in .tiff named as pristine TiGr5 ELI; laser TiGr5 ELI; pristine TiNbZr; laser TiNbZr; pristine TiNbSnTa; laser TiNbSnTa
		
	- Data description of:  Methods used for acquiring/creation of data: energy-dispersive X-ray spectroscopy (EDS, analyzer X-ManN, 20 mm2 SDD detector)
				Methods used for data processing: data were processed as graph containing concentration of elements in at.% using MS Excel 2016 (report from EDS software) and then Adobe Photoshop 8.0
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-   Analysis 3	(AFM)
		- Folder containing scans of surface samples named as TiGr5ELI  laser_1_mikron_f1; TiGr5ELI  laser_30_mikronu_f1; TiGr5ELI_pristine_1_mikron_f1; TiGr5ELI_pristine_30_mikronu_f1;
                                                                      TiNbSnTa  laser_1_mikron_f1; TiNbSnTa  laser_30_mikronu_f1; TiNbSnTa  pristine_1_mikron_f1; TiNbSnTa  pristine_30_mikronu_f1;
                                                                      TiNbZr_laser_1_mikron_f1; TiNbZr_laser_30_mikronu_f1; TiNbZr_pristine_1_mikron_f1; TiNbZr_pristine_30_mikronu_f1

	- Data description of:  Methods used for acquiring/creation of data: atomic force microscopy (Dimension ICON device)
				Methods used for data processing: NanoScope® analysis programme, using "Flatten" method and "Adjust Image Color Scale"
				Summary of variables: Ra = Average Surface Roughness;			
				
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-   Analysis 4	(XPS)
		- Data are in .tiff named as laser TiNbZr; pristine TiNbZr
                - Data are in .xls named as laser TiGr5ELI.deconvoluted.Oxygen; TiGr5ELI.deconvoluted.Ti; TiGr5ELI.LASER.deconvoluted.Oxygen; TiGr5ELI.LASER.deconvoluted.Ti
		
	- Data description of:  Methods used for acquiring/creation of data: X-Ray Photoelectron Spectroscopy (ESCAProbeP, An Omicron Nanotechnology)
				Methods used for data processing: data were processed by CasaXPS software and exported as .tiff, and data as .xls
				
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-   Analysis 5	(Antibacterial activity) 
                - Data are in .xlsx named as Figure9.Antibacterial.SEpidermidis; Figure10.Antibacterial.EColi
               
			- Data description of: The antibacterial activity of pristine and laser-treated samples was measured using the drop plate method against two bacterial strains: the Gram-positive and Gram-negative bacteria    
                                               Staphylococcus epidermidis (DBM 2124) and Escherichia coli (DBM 3138), respec-tively. Overnight cultures (16 h) of both strains were grown in Luria-Bertani (LB; Merck, USA) medium at 37 °C    
                                               with shaking at 200 rpm. The optical density at 600 nm (OD600) of each culture was measured and adjusted to OD600 = 1 using prewarmed (37 °C) phos-phate-buffered saline (PBS). The bacterial  
                                               suspensions were then further diluted to 4 x 104 and 2 x 104 colony-forming units (CFU) per mL for S. epidermidis and E. coli, respectively. Aliquots of these suspensions were incubated 
                                               with the tested samples at 37 °C for 3 and 6 h. Then, 25-µL drops were pipetted onto LB agar plates (5 drops per sample, three repli-cates) and incubated at 37 °C for 24 h. The resulting 
                                               CFU numbers on plates from each sample were determined and averaged. Bacterial suspensions incubated in PBS without samples served as controls.
                          Methods used for data processing: data were processed as graph containing concentration of elements in at.% using MS Excel 2016 
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-   Analysis 6	(Cytocompatibility)
                - Data are in .xlsx named as Figure11.Cell.Viability
               
			- Data description of: The cytocompatibility of the tested samples was evaluated using human osteosar-coma cells (U-2 OS; ATCC, USA). Cells were cultured at 37 °C, 5% CO2, and a humidified atmosphere in high- 
                                               glucose Dulbecco's modified Eagle's medium (DMEM; Merck, USA) with 10% fetal bovine serum (FBS; Merck, USA). Cells were passaged three times per week using trypsin-EDTA and maintained in  
                                               the exponential growth phasefunction, averages values and SD (standard deviation) were data were processed as graphs containing number of cells. 
                          Methods used for data processing: data were processed as graph containing concentration of elements in at.% using MS Excel 2016 
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