Number of the records: 1
A possible role for short introns in the acquisition of stroma-targeting peptides in the flagellate Euglena gracilis
Title A possible role for short introns in the acquisition of stroma-targeting peptides in the flagellate Euglena gracilis Author info Matej Vesteg ... [et al.] Author Vesteg Matej 1982- (40%)
Co-authors Vacula Rostislav (10%)
Steiner Jürgen M. (5%)
Mateášiková Blanka (5%)
Loeffelhardt Wolfgang (10%)
Brejová Broňa (5%)
Krajčovič Juraj (25%)
Source document DNA research. Vol. 17, no. 4 (2010), pp. 223-231. - Oxford : Oxford University Press, 2010 Keywords fotosystémy - photosystems chloroplasty - chloroplasts proteíny - proteins trypanozóma spavičná - Trypanosoma brucei genómy - genomes Language English Country Great Britian systematics 577 Annotation The chloroplasts of Euglena gracilis bounded by three membranes arose via secondary endosymbiosis of a green alga in a heterotrophic euglenozoan host. Many genes were transferred from symbiont to the host nucleus. A subset of Euglena nuclear genes of predominately symbiont, but also host, or other origin have obtained complex presequences required for chloroplast targeting. This study has revealed the presence of short introns (41-93 bp) either in the second half of presequence-encoding regions or shortly downstream of them in nine nucleus-encoded E. gracilis genes for chloroplast proteins (Eno29, GapA, PetA, PetF, Pet), PsaF, PsbM, PsbO, and PsbW). In addition, the E. gracilis Pbgd gene contains two introns in the second half of presequence-encoding region and one at the border of presequence-mature peptide-encoding region. Ten of 12 introns present within presequence-encoding regions or shortly downstream of them identified in this study have typical eukaryotic GT/AG borders, are T-rich, 45-50 bp long, and pairwise sequence identities range from 27 to 61%. Thus single recombination events might have been mediated via these cis-spliced introns. A double crossing over between these cis-spliced introns and trans-spliced introns present in 5'-UTRs of Euglena nuclear genes is also likely to have occurred. Thus introns and exon-shuffling could have had an important role in the acquisition of chloroplast targeting signals in E. gracilis. The results are consistent with a late origin of photosynthetic euglenids. URL Priamy link Public work category ADC No. of Archival Copy 37275 Repercussion category DOBÁKOVÁ, Eva - FLEGONTOV, Pavel - SKALICKÝ, Tomáš - LUKEŠ, Július. Unexpectedly streamlined mitochondrial genome of the euglenozoan Euglena gracilis. In Genome biology and evolution. ISSN 1759-6653, 2015, vol. 7, no. 12, pp. 3358-3367.
MILANOWSKI, Rafal - GUMINSKA, Natalia - KARNKOWSKA, Anna - ISHIKAWA, Takao - ZAKRYS, Bozena. Intermediate introns in nuclear genes of euglenids are they a distinct type? In BMC evolutionary biology. ISSN 1471-2148, 2016, vol. 16, art. no. 49.
VANCLOVÁ, Anna M. G. - HADARIOVÁ, Lucia - HRDÁ, Štěpánka - HAMPL, Vladimír. Secondary plastids of euglenophytes. In Secondary endosymbioses. ISSN 0065-2296, 2017, vol. 84, pp. 321-358.
BICUDO, Carlos E. de M. - MENEZES, Mariangela. Phylogeny and classification of euglenophyceae : a brief review. In Frontiers in ecology and evolution. ISSN 2296-701X, 2016, vol. 4, article no. 17, pp. 1-15.
SHEINER, Lilach - STRIEPEN, Boris. Protein sorting in complex plastids. In Biochimica et biophysica acta : molecular cell research. ISSN 0167-4889, 2013, vol. 1833, no. 2, special issue, pp. 352-359.
MILANOWSKI, Rafal - KARNKOWSKA, Anna - ISHIKAWA, Takao - ZAKRYS, Bozena. Distribution of conventional and nonconventional introns in tubulin (alpha and beta) genes of euglenids. In Molecular biology and evolution. ISSN 0737-4038, 2014, vol. 31, no. 3, pp. 584-593.
BURKI, Fabien - HIRAKAWA, Yoshihisa - KEELING, Patrick J. Intragenomic spread of plastid-targeting presequences in the coccolithophore emiliania huxleyi. In Molecular biology and evolution. ISSN 0737-4038, 2012, vol. 29, no. 9, pp. 2109-2112.
HRDÁ, Štěpánka - FOUSEK, Jan - SZABOVÁ, Jana - HAMPL, Vladimír V. - VLČEK, Čestmír. The plastid genome of eutreptiella provides a window into the process of secondary endosymbiosis of plastid in euglenids. In Plos one. ISSN 1932-6203, 2012, vol. 7, no. 3, pp. 1-10.
TAMAKI, Shun - NISHINO, Kohei - OGAWA, Takahisa - MARUTA, Takanori - SAWA, Yoshihiro - ARAKAWA, Kazuharu - ISHIKAWA, Takahiro. Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions. In PloS one. ISSN 1932-6203, 2019, vol. 14, no. 12, pp. 1-13.
GUMINSKA, Natalia - ZAKRYS, Bozena - MILANOWSKI, Rafal. A new type of circular RNA derived from nonconventional introns in nuclear genes of Euglenids. In Journal of molecular biology. ISSN 0022-2836, 2021, vol. 433, no. 3, pp. 1-9.
SOUKAL, Petr - HRDA, Stepanka - KARNKOWSKA, Anna - MILANOWSKI, Rafal - SZABOVA, Jana - HRADILOVA, Miluse - STRNAD, Hynek - VLCEK, Cestmir - CEPICKA, Ivan - HAMPL, Vladimir. Heterotrophic euglenid Rhabdomonas costata resembles its phototrophic relatives in many aspects of molecular and cell biology. In Scientifc reports. ISSN 2045-2322, 2021, vol. 11, no. 1, pp. 1-17.
Database xpca - PUBLIKAČNÁ ČINNOSŤ 
unrecognised
Number of the records: 1